Methylation Profile


Indications:

This test assesses metabolism of the essential amino acid methionine (Met). Methionine is paramount in
two metabolic processes; (1) transmethylation that is critical for the methylation of hundreds of important
molecules such as DNA, RNA, proteins, neurotransmitters and membrane phosphatidylcholine, and (2)
transsulfurration that leads to the biosynthesis of cysteine and hence glutathione , both of which have many
important protective / detoxification functions. Aberrant Met metabolism can be caused by nutritional
deficiencies, exposures to environmental toxicants and/or genetic polymorphisms and can have significant
adverse health consequences. Identification of such abnormalities can guide appropriate nutritional
intervention towards normalization of methionine metabolism and decreased risk and incidence of adverse
health effects.

The amino acids and intermediary amino acid metabolites were measured by liquid chromatography - mass
spectrometry. Reference values are age and sex specific. If patient values deviate from normal,
comprehensive descriptive paragraphs will be presented as part of the test report.

Patient Instructions:

Patients need to attend Biolab for this test as the blood samples have to be centrifuged and the samples immediately frozen.
Overnight fast and avoid methionine, cysteine, and SAMe supplements for 48 hours prior to the test.

Appointment Notes:

Appointments available up to 4:30pm Monday to Thursday and 2pm Fridays

Datasheet:

methylddi.pdf (Click to Download)

Sample Report:

methprof.pdf (Click to Download)

Sample Requirements:

Use Doctor's Data kit (transfer EDTa blood to two supplied tubes & mix).
Samples should be separated promptly (within 15 minutes) and frozen within 45 minutes.

Postal Samples Acceptable:

Yes

For further details please contact the laboratory at: lab@xxxxbiolab.co.uk